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Figure 4.34 Fluorescence spectroscopy of single biomolecules (A) Localization of a macromolecule labeled with a single uorophore F with nanometer accuracy. The point-spread-function (PSF) can be localized within a few tenths of a nanometer. (B) Colocalization of two macromolecules labeled with two noninteracting uorophores, F1 and F2. Their distance can be measured by subtracting the center positions of the two PSFs. (C) Intramolecular detection of conformational changes by spFRET. D and A are donor and acceptor; ID and IA are donor and acceptor emission intensities; t is time. (D) Dynamic colocalization and detection of association or dissociation by intermolecular spFRET. Donor and acceptor intensities are anticorrelated both in (C) and (D). (E) The orientation of a single immobilized dipole can be determined by modulating the excitation polarization. The uorescence emission follows the angle modulation. (F) The orientational freedom of motion of a tethered uorophore can be measured by modulating the excitation polarization and analyzing the emission at orthogonal s and p polarization detectors. IS and IP are emission intensities of s and p detectors. (G) Ion channel labeled with a uorescence indicator I. Fluctuations in its intensity I report on local ion concentration changes. (H) Combination of (C) and (G). D and A report on conformational changes whereas I reports on ion ux (Reproduced from Weiss, 1999, Fig. 1) Labeling schemes (left) and physical observables (right).

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import actionscriptbible.drawing.DrawingUtilities; import flash.geom.Matrix; var var var var mSelected:MovieClip; mCurrent:MovieClip; nInterval:Number; oStartPoint:Object;

The phenomenal charge charge recognition by H2A, H2B, H3 and H4 is largely responsible for the extraordinary capacity of these proteins to distort the DNA helical axis from linear to bent with a high degree of curvature However, little remains known about the molecular processes of assembly and disassembly of chromatin laments after and before gene expression events In DNA terms, the other great triumph of molecular recognition and binding is the control of gene expression through DNA-sequence selective protein binding Proteins that bind DNA and control expression are known as transcription factors One such is the GCN4 protein of yeast that is perhaps the rst to be studied in molecular detail (Figure 78) The extraordinary fact is that DNA has impressively few genes in the total length, especially where eukaryotes are concerned.

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DBF is the dBASE III and dBASE IV (also known as xBASE) database format. Field names can be no more than 10 characters long, with a maximum of 254 characters

1. Add about 50 mL of starch indicator to the vessel. 2. Add suf cient iodine solution to the vessel to produce an intense black color. Note the quantity used. 3. With the agitator running at the desired speed, quickly incorporate sodium thiosulfate into the vessel at a rate approximately 2.5 times the quantity of iodine solution used. As mixing progresses, the liquid will clarify. 4. After each test, slowly add iodine solution until the neutral point is again reached, demonstrated by a hint of light blue appearing. The test can then be repeated. It should be noted that this technique must be used with care with solutions of CMC, which have a tendency to decolorize iodine solutions over time, albeit slowly.

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he CD-ROM included with this book aids you with many examples and tutorials by providing many useful files, including the following: Trial versions of Macromedia Flash 5, Dreamweaver 4, Fireworks 4, FreeHand 9, and Director 8. Evaluation versions of many .SWF-compatible applications and utilities, including Swift3D, SWiSH, SwiftInspector, and Swift-Generator. Demo versions of Sonic Foundry s audio applications, Sound Forge 4.5, and ACID Pro 2. Limited-edition version of Joey Lott s sendmail.cgi Perl script, to be used in conjunction with the Flash form lesson in 24. Just about every .FLA and .SWF file that is discussed in the book, including those used in Expert Tutorials. QuickTime movies and QTVR panorama and object movies. Many thanks to Paul Nykamp from Focus VR for the QTVR samples.

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