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of individual microbial cells without cultivation. Microbiol. Rev. 59:143 169. Amann, R., N. Springer, W. Ludwig, H.-D. G rtz, and K.-H. Schleifer. 1991. Identi cation in situ and phylogeny of uncultured bacterial endosymbionts. Nature 351:161 164. Andrews, J.E. 2004. An Introduction to Environmental Chemistry. Blackwell Science, Oxford. Atlas, R.M. and R. Bartha. 1998. Microbial Ecology: Fundamentals and applications, 4th edn. Benjamin Cummings, Menlo Park, CA. Baxevanis, A.D. and B.F.F. Ouellette. 2001. Bioinformatics: A practical guide to the analysis of genes and proteins, 2nd edn. Wiley Interscience, New York. Bitton, G.E. 1999. Wastewater Microbiology, 2nd edn. Wiley Liss, New York. Boetius, A., K. Ravenschlag, C.J. Schubert, et al. 2000. A marine microbial consortium apparently mediating anaerobic oxidation of methane. Nature 407:623 626. Boxall, A.B.A., C.J. Sinclair, K. Fenner, D. Kolpin, and S.J. Maund. 2004. When synthetic chemicals degrade in the environment. Environ. Sci. Technol. 38:368A 375A. Brady, N.C. and R.R. Weil. 1999. The Nature and Properties of Soils, 12th edn. Prentice Hall, Upper Saddle River, NJ. Brock, T.D. 1961. Milestones in Microbiology. Prentice Hall, Englewood Cliffs, NJ. Bull, I.D., N.R. Parekh, G.H. Hall, P. Ineson, and R.P. Evershed. 2000. Detection and classi cation of atmospheric methane oxidizing bacteria in soil. Nature 405:175 178. Burlage, R., R. Atlas, D. Stahl, G. Geesey, and G. Sayler (eds) 1998. Techniques in Microbial Ecology. Oxford University Press, New York. Chapin, F.S., P.A. Matson, and H.A. Mooney. 2002. Principles of Terrestrial Ecosystem Ecology. Springer Verlag, New York. Christian, G.D. 2003. Analytical Chemistry, 6th edn. Wiley and Sons, New York. Coates, J.D. and L.A. Achenbach. 2004. Microbial perchlorate reduction: rocket-fuelled metabolism. Nature Rev. Microbiol. 2:569 580. Conrad, R. 1996. Soil microorganisms as controllers of atmospheric trace gases (H2, CO, CH4, OCS, N2O, and NO). Microbiol. Rev. 60:609 640.

Working hand-in-hand with selecting an appropriate file type is the other main goal of exporting, referred to as optimization. Optimization is the process of producing the best looking, smallest possible file. An optimized image loads faster, without sacrificing perceived quality. Because data is thrown away in the optimization process, avoid opening and editing the so-called public file types, shown in Figure 15-1, whenever possible. Store your work and move it between applications using the private file types. Think of the private file types as originals, and the public file types as photocopies made for sharing. Optimizing and exporting in Fireworks is focused around the preview tabs of the document window, the Optimize panel, and the Frames panel for animations. Adjusting settings in these panels is a necessary precursor to choosing File Export to create your exported image file. After you ve made these settings, they are saved with your Fireworks PNG file for next time. Export settings are as much a part of a document as the kinds of fills or strokes you used. The hardest part of optimizing is finding a balance between image quality and file size. Fireworks takes a lot of the guesswork out of this task by providing up to four comparison views of different formats at various color resolutions or compressions.

One limitation that must be kept in mind, however, is the de ciency of molecular methods in analyzing heterogeneous or mosaic cell populations 133 Identity Testing 1331 Con rmation of Species of Origin Isoenzyme analysis is based on measuring the charge-to-mass ratio of different isoenzyme activities using an agarosebased gel to separate the various polymorphic enzymes that can be identi ed for even just one enzyme reactivity The cells are lysed, and the released enzymes are stabilized.

All the examples in this chapter, including the Trying It Out in the Real World exercise, utilize the SQL query and PivotTable report outlined in this section. If you plan to

When you first open a page in Dreamweaver, your default Web page is untitled, with no background image and only a plain, white background. You can change any of these properties and more through Dreamweaver s Page Properties dialog box.

The eyeball icon to the left of the layer name allows you to show or hide the objects on the layer. Clicking the icon for a layer shows or hides all of its sublayers, but each sublayer has its own visibility icon as well. The empty box to the right of the eye visibility icon allows you to lock a layer or sublayer. Once locked, the objects on the layer cannot be selected or modified in any way. As with visibility, you can lock a layer and thus lock all of its sublayers, or you can lock individual sublayers as needed.

In this rudimentary example, with only two state variables in play and a basic branching structure, you can notice an improvement in readability because intelligible variable names replace function calls and potentially convoluted expressions throughout the script s body The work of retrieving parameter and environment data is performed once at the script s commencement, and the variables are then available throughout the course of the script Overall, structuring your scripts in this way offers potential improvements in n Efficiency: Functions and calculations determining state need be evaluated only once and thereafter variables are available to be referenced Variables are held in memory; referencing uses minimal resources n Reliability: Given values are determined once and remain in memory throughout, so if a script action changes the state of your solution, the execution of the script s logic is not impacted.